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Cell population definition according to surface marker.

Journal: Scientific Reports

Article Title: Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential

doi: 10.1038/s41598-024-73875-x

Figure Lengend Snippet: Cell population definition according to surface marker.

Article Snippet: Human Dermal Endothelial Cells (EC) from juvenile donor (3yo) were purchased from Promocell and cultivated in EGM-2 (PromoCell, Heidelberg, Germany).

Techniques: Marker

Quantification of cell populations in the SVF and their repartition among CD45- and CD45 + populations. n = 6 donors were used in each age category. * p value < 0,05 indicated in bold . Unpaired Wilcoxon test was used to test the significance of ASC and pericytes populations. Unpaired student t-test was used to test the significance of other cell populations.

Journal: Scientific Reports

Article Title: Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential

doi: 10.1038/s41598-024-73875-x

Figure Lengend Snippet: Quantification of cell populations in the SVF and their repartition among CD45- and CD45 + populations. n = 6 donors were used in each age category. * p value < 0,05 indicated in bold . Unpaired Wilcoxon test was used to test the significance of ASC and pericytes populations. Unpaired student t-test was used to test the significance of other cell populations.

Article Snippet: Human Dermal Endothelial Cells (EC) from juvenile donor (3yo) were purchased from Promocell and cultivated in EGM-2 (PromoCell, Heidelberg, Germany).

Techniques:

( A ) Diversity of sprouting of endothelial cells from dextran beads stained with phalloidin and Hoechst to visualize actin and nucleus, respectively (original magnification 10x, bar, 200 μm). ( B ) Total sprouts length with co-culture of EC with ASC (EC/ASC; dark grey) and fibroblasts (EC/Fb; light grey) as stimulating cells. ( B ) Total sprouts length with co-culture of EC with either ASC (EC/ASC; dark grey) and fibroblasts (EC/Fb; light grey) as stimulating cells. ( C ) Total sprouts length according to co-culture of EC with ASC from young (EC/ASC y; blue) or old (EC/ASC o; red) donors as stimulating cells. n = 5 donors for ASC young, n = 6 donors for ASC old, n = 1 donor for fibroblasts, experiments were conducted in 4 replicates. Unpaired Wilcoxon test was used to test the significance. * P value < 0.05.

Journal: Scientific Reports

Article Title: Deciphering influence of donor age on adipose-derived stem cells: in vitro paracrine function and angiogenic potential

doi: 10.1038/s41598-024-73875-x

Figure Lengend Snippet: ( A ) Diversity of sprouting of endothelial cells from dextran beads stained with phalloidin and Hoechst to visualize actin and nucleus, respectively (original magnification 10x, bar, 200 μm). ( B ) Total sprouts length with co-culture of EC with ASC (EC/ASC; dark grey) and fibroblasts (EC/Fb; light grey) as stimulating cells. ( B ) Total sprouts length with co-culture of EC with either ASC (EC/ASC; dark grey) and fibroblasts (EC/Fb; light grey) as stimulating cells. ( C ) Total sprouts length according to co-culture of EC with ASC from young (EC/ASC y; blue) or old (EC/ASC o; red) donors as stimulating cells. n = 5 donors for ASC young, n = 6 donors for ASC old, n = 1 donor for fibroblasts, experiments were conducted in 4 replicates. Unpaired Wilcoxon test was used to test the significance. * P value < 0.05.

Article Snippet: Human Dermal Endothelial Cells (EC) from juvenile donor (3yo) were purchased from Promocell and cultivated in EGM-2 (PromoCell, Heidelberg, Germany).

Techniques: Staining, Co-Culture Assay